Name | pyruvate kinase from rabbit muscle |
Synonyms | EC 2.7.1.40 pyruvate phosphoferase pyruvate kinase from rabbit muscle |
CAS | 9001-59-6 |
EINECS | 232-616-2 |
Water Solubility | Soluble in water ( 1mg/ml). |
Appearance | powder |
Color | white |
Storage Condition | -20°C |
MDL | MFCD00081946 |
Physical and Chemical Properties | Glycerol suspended in 50% |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-21 |
TSCA | Yes |
HS Code | 35079090 |
Reference Show more | 1. [IF = 3.772] Y. Duan et al."Antifgal mechanism of Streptomyces ma. FS-4." J Appl Microbiol. 2021 Jan;130(1):196-207 Note: For some products, we can only provide some information, and we do not guarantee the authority of the information provided, for customer reference and communication purposes only. use: This product is for scientific research only, not for other purposes. storage conditions: -20 °c Appearance: white powder Thermal stability < 60 °c (pH 8.5, 20min) pH Stability 5.0-10.0 (37 ℃, 20hr) optimal temperature: 65 ℃ optimal pH: 7.5 Michaelis constant: 1.1mM (ADP) 2.2 (PEP) Isoelectric point :5.2 Molecular weight: 68 kDa (SDS-PAGE) Unit definition: One unit will convert one micromole of phospho-enol pyruvent to pyruvent min at pH 7.2 at 30 ° C. Specific activity: ≥ 200U/mg protein. |
suspended in 50% glycerol.
commonly used diagnostic enzymes. Biochemical reaction and enzyme analysis, quantitative determination of phosphoenolpyruvate (PEP), ADP, blood glucose, triglyceride and other enzymes and some catalytic role of phosphorus transfer.
1mL (200u) packaging, sealed 0~4 deg C storage.
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Introduction | pyruvate kinase is a key enzyme in the glycolysis pathway that catalyzes the conversion of phosphoenolpyruvate to enol pyruvate, and produce ATP, so it has an important physiological role. Pyruvate kinase is widely distributed in the human body, there are four kinds of isoenzymes, divided into L, R, M1 and M2. L type only exists in liver parenchyma, kidney and small intestinal mucosa, R type only exists in mature red blood cells, M1 type exists in brain and muscle, M2 type except skeletal muscle, it is widely distributed in various tissues, especially in kidney and malignant tumor cells. In normal adult liver is mainly L-type, and in the fetus is mainly R-type. |
metabolism | pyruvate kinase is mainly involved in the regulation of two major metabolic pathways, it catalyzes the formation of pyruvate from phosphoenolpyruvate and releases 2 molecules of ATP, which is irreversible under physiological conditions. Second, it participates in the regulation of genetic material metabolism and catalyzes the exchange of phosphate groups. |
Use | commonly used diagnostic enzymes. Biochemical reactions and enzyme analysis. Quantitative Determination of phosphoenolpyruvate (PEP), ADP, blood glucose, triglycerides, and some of the catalytic role of enzymes in the transfer of phosphorus. used in biochemical studies, clinically used as a diagnostic enzyme for the determination of adenosine diphosphate (ADP), adenosine monophosphate (AMP), phosphoenolpyruvate and phosphoglycerate. |
production method | using rabbit muscle as raw material, homogenate, water extraction, ammonium sulfate and ethanol precipitation, after the inactive protein was removed by heating, it was precipitated with ammonium sulfate again, and purified by recrystallization. |